✦ LIBER ✦

Microarray analysis of 1,25(OH)2D3 regulated gene expression in human primary osteoblasts

✍ Scribed by Paola Tarroni; Isabella Villa; Emanuela Mrak; Francesca Zolezzi; Michela Mattioli; Claudio Gattuso; Alessandro Rubinacci

Publisher: John Wiley and Sons
Year: 2012
Tongue: English
Weight: 448 KB
Volume: 113
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.23392

No coin nor oath required. For personal study only.

✦ Synopsis

Though extensive studies have been conducted, questions regarding the molecular effectors and pathways underlying the regulatory role of 1,25(OH) 2 D 3 in human osteoblasts other than cell differentiation and matrix protein production remain unanswered. This study aims to identify genes and pathways that are modulated by 1,25(OH) 2 D 3 treatment in human osteoblasts. Primary osteoblast cultures obtained from human bone tissue samples were treated with 1,25(OH) 2 D 3 (10 À7 M) for 24 h and their transcritptomes were profiled by microarray analysis using the Affymetrix GeneChip 1 . Statistical analysis was conducted to identify genes whose expression is significantly modulated following 1,25(OH) 2 D 3 treatment. One hundred and fifty-eight genes were found to be differentially expressed. Of these, 136 were upregulated, indicating clear transcriptional activation by 1,25(OH) 2 D 3 . Biostatistical evaluation of microarray data by Ingenuity Pathways Analysis (IPA) revealed a relevant modulation of genes involved in vitamin D metabolism (CYP24), immune functions (CD14), neurotransmitter transporters (SLC1A1, SLC22A3), and coagulation [thrombomodulin (THBD), tissue plasminogen activator (PLAT), endothelial protein C receptor (PROCR), thrombin receptor (F2R)]. We identified a restricted number of highly regulated genes and confirmed their differential expression by real-time quantitative PCR (RT qPCR). The present genome-wide microarray analysis on 1,25(OH) 2 D 3 -treated human osteoblasts reveals an interplay of critical regulatory and metabolic pathways and supports the hypothesis that 1,25(OH) 2 D 3 can modulate the coagulation process through osteoblasts, activates osteoclastogenesis through inflammation signaling, modulates the effects of monoamines by affecting their reuptake. J.

📜 SIMILAR VOLUMES

Microarray-based gene expression analysi

Microarray-based gene expression analysis of human osteoblasts in response to different biomaterials

✍ Karina F. Bombonato-Prado; Larissa S. Bellesini; Cristina M. Junta; Márcia M. Ma 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 148 KB

## Abstract Several biomaterials have been widely used in bone regeneration/substitution procedures in orthopedic and oral surgery. However, how these biomaterials alter osteoblast gene expression is poorly understood. We therefore attempted to address this question by using cDNA microarray techniq

1?,25(OH)2D3 Regulation of integrin expr

1?,25(OH)2D3 Regulation of integrin expression is substrate dependent

✍ Raz, P. ;Lohmann, C. H. ;Turner, J. ;Wang, L. ;Poythress, N. ;Blanchard, C. ;Boy 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 366 KB

## Abstract Osteoblasts are attachment‐dependent cells that interact with their surface through integrin‐mediated mechanisms. Their differentiation is regulated by 1,25‐dihydroxyvitamin D3 [1α,25(OH)~2~D~3~] and is affected by substrate chemistry and microtopography, suggesting that 1α,25(OH)~2~D~3

Differential regulation of Cbfa1/Runx2 a

Differential regulation of Cbfa1/Runx2 and osteocalcin gene expression by vitamin-D3, dexamethasone, and local growth factors in primary human osteoblasts

✍ Volker Viereck; Heide Siggelkow; Simone Tauber; Dirk Raddatz; Norbert Schutze; M 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 126 KB 👁 1 views

Core binding factor alpha 1 (Cbfa1) is an osteoblast-specific transcription factor essential to develop a mature osteoblast phenotype. However, its exact role in the signaling of various osteotropic-differentiating agents is still unclear. In this study, we assessed the effects of 1,25-(OH)(2)-D3 (D

Evidence for regulation of amelogenin ge

Evidence for regulation of amelogenin gene expression by 1,25-dihydroxyvitamin D3 in vivo

✍ Petros Papagerakis; Dominique Hotton; Frederic Lezot; Steve Brookes; William Bon 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 437 KB 👁 1 views

The unique hereditary enamel defect clearly related to the disturbance of one enamel matrix protein is X-linked amelogenesis imperfecta (AI), in which several mutations of amelogenin gene have been identified. The clinical phenotype of many of these subjects shows similarities with enamel defects re

Hormonal regulation of [Ca2+]i in perios

Hormonal regulation of [Ca2+]i in periosteal-derived osteoblasts: effects of parathyroid hormone, 1,25(OH)2D3 and prostaglandin E2

✍ M.A.A. Said Ahmed; L.M. Walker; S.J. Publicover; A.J. El Haj 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 208 KB 👁 2 views

The effects of hormonal modulators of osteoblast function, parathyroid hormone, 1,25(OH) 2 D 3 and prostaglandins on [Ca 2ϩ ] i in periosteal-derived osteoblasts from rat femurs have been investigated. Our results show that application of parathyroid hormone PTH (10 Ϫ5 M) and prostaglandin E 2 (PGE

1,25-dihydroxyvitamin D3 regulates the e

1,25-dihydroxyvitamin D3 regulates the expression of VDR and NGF gene in Schwann cells in vitro

✍ Anne Cornet; Christel Baudet; Isabelle Neveu; Annick Baron-Van Evercooren; Phili 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 69 KB 👁 2 views

The vitamin D receptor (VDR) is a nuclear receptor that mediates the effect of the active metabolite of vitamin D3, the 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3). To investigate the potential role of this hormone in the peripheral nervous system, we have studied the VDR expression in Schwann cells. Th