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Hormonal regulation of [Ca2+]i in periosteal-derived osteoblasts: effects of parathyroid hormone, 1,25(OH)2D3 and prostaglandin E2

✍ Scribed by M.A.A. Said Ahmed; L.M. Walker; S.J. Publicover; A.J. El Haj

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 208 KB
Volume: 183
Category: Article
ISSN: 0021-9541
DOI: 10.1002/(sici)1097-4652(200005)183:2<163::aid-jcp3>3.0.co;2-t

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✦ Synopsis

The effects of hormonal modulators of osteoblast function, parathyroid hormone, 1,25(OH) 2 D 3 and prostaglandins on [Ca 2ϩ ] i in periosteal-derived osteoblasts from rat femurs have been investigated. Our results show that application of parathyroid hormone PTH (10 Ϫ5 M) and prostaglandin E 2 (PGE 2 ) (4 M) result in a rapid heterogeneous elevation in [Ca 2ϩ ] i that, in the case of PTH, is dependent on both extracellular and intracellular sources of calcium. Variable responses to treatments have been found within populations of cells. The PGE 2 response is dose dependent. Treatment with 1,25(OH) 2 D 3 (10 Ϫ8 M) induces a brief (60 -90 sec) elevation in [Ca 2ϩ ] i that is almost totally abolished in EGTA-buffered Ca 2ϩfree medium. Interactive effects of multiple hormone treatments have been observed. Pretreatment with 1,25(OH) 2 D 3 results in near-total inhibition of the PTH and PGE 2 responses. In conclusion, modulation of [Ca 2ϩ ] i appears to play a role not only in the direct effects of osteotropic hormones on osteoblasts but also in the synergistic and antagonistic effects between circulating hormones.

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