Background: Activation-induced protein phosphorylation can be studied by Western blotting, but this method is time consuming and depends on the use of radioactive probes for quantitation. We present a novel assay for the assessment of protein phosphorylation based on latex particles and flow cytomet
Microaffinity Columns for Analysis of Protein–Protein Interactions
✍ Scribed by S. Kussmann-Gerber; I. Kratchmarova; S. Mandrup; K. Kristiansen
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 91 KB
- Volume
- 271
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
approximately 40 pg, more sensitive detection system (for example, fluorescence) is needed to monitor extremely low amounts in the samples.
Among three CE running buffers in IAA analysis, buffer I gives the best separation, although it has a limited buffering capacity. It should be pointed out that fresh CE running buffer is important for a good separation and reliability.
Acknowledgments.
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