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MGMTpromoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

✍ Scribed by Annette Bentsen Håvik; Petter Brandal; Hilde Honne; Hanne-Sofie Spenning Dahlback; David Scheie; Merete Hektoen; Torstein Ragnar Meling; Eirik Helseth; Sverre Heim; Ragnhild A Lothe; Guro Elisabeth Lind


Book ID
115020912
Publisher
BioMed Central
Year
2012
Tongue
English
Weight
307 KB
Volume
10
Category
Article
ISSN
1479-5876

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## Abstract Methylation of CpG dinucleotides in the promoter sequence of a gene can lead to deregulated and suppressed gene expression. In this study, we have developed procedures for methylation‐specific polymerase chain reaction (MSP) and sequencing analysis to determine CpG methylation status of