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Methylation of cystatin M promoter is associated with unfavorable prognosis in operable breast cancer

✍ Scribed by Magdalini Kioulafa; Ioanna Balkouranidou; Georgia Sotiropoulou; Loukas Kaklamanis; Dimitris Mavroudis; Vassilis Georgoulias; Evi S. Lianidou

Publisher
John Wiley and Sons
Year
2009
Tongue
French
Weight
158 KB
Volume
125
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The methylation status of cystatin M (CST6) gene in breast tumors was investigated and its prognostic significance as a novel breast cancer biomarker was evaluated. Using methylation‐specific PCR (MSP), CST6 promoter methylation was examined in 134 formalin fixed paraffin‐embedded tissues (FFPEs): 10 pairs of breast tumors and their surrounding normal tissues, 10 breast fibroadenomas, 11 normal breast tissues and 93 breast tumors. Methylation of CST6 promoter was observed in 2/21 (9.5%) noncancerous breast tissues, 1/10 (10%) benign breast tumors (fibroadenomas) and 52 (55.9%) operable breast cancer tumor samples. CST6 was rarely methylated in the normal tissue surrounding the tumor (10%). During the follow‐up period, 24 (25.8%) patients relapsed and 19 (20.4%) died. CST6 methylation was detected in 19 (79.2%) of patients who relapsed and in 15 (78.9%) of patients who died. Disease‐free‐interval (DFI) and overall survival (OS) were significantly associated with CST6 promoter methylation (p = 0.004 and p = 0.001 respectively). Multivariate analysis revealed that CST6 methylation is an independent prognostic factor for DFI (HR = 3.484; 95% CI: 1.155–10.511; p = 0.027). and OS (HR = 9.190; 95% CI: 1.989–42.454; p = 0.004). CST6 promoter methylation status in tumor cells seems to provide important prognostic information in operable breast cancer and merits to be further evaluated and validated in a larger cohort of patients. © 2009 UICC

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