Methods of Protein Separation

✍ Scribed by Gary K. Ackers (auth.), Nicholas Catsimpoolas (eds.)

• Publisher: Springer US
• Year: 1976
• Tongue: English
• Leaves: 338
• Series: Biological Separations
• Edition: 1
• Category: Library

✦ Synopsis

This open-end treatise on methods concerning pro tein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles för achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaining insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection and/or modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of pro teins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area. Nicholas Catsimpoolas Cambridge, Massachusetts vü CONTENTS Contents of Volume 1 . xvii Chapter 1 Scanning Gel Cbromatography Gary K. Ackers I.

✦ Table of Contents

Front Matter....Pages i-xviii
Scanning Gel Chromatograpy....Pages 1-26
Analytical and Preparative Polyacrylamide Gel Electrophoresis....Pages 27-144
Estimation of Molecular Weight by Gel Filtration and Gel Electrophoresis....Pages 145-179
Estimation of Molecular Weight by Gel Filtration and Gel Electrophoresis....Pages 181-218
Zone Electrophoresis, Isoelectric Focusing, and Displacement Electrophoresis (Isotachophoresis) in Carrier-Free Solution....Pages 219-231
Hydrophobic Interaction Chromatography of Proteins on Neutral Adsorbents....Pages 233-243
Hydrophobic Adsorption Chromatography of Proteins....Pages 245-278
Sievorptive Chromatography....Pages 279-319
Back Matter....Pages 321-326

✦ Subjects

Biochemistry, general