Methods for Affinity-Based Separations of Enzymes and Proteins

✍ Scribed by Munishwar N. Gupta, Ipsita Roy (auth.), Prof. Dr. Munishwar Nath Gupta (eds.)

• Publisher: Birkhäuser Basel
• Year: 2002
• Tongue: English
• Leaves: 234
• Series: Methods and Tools in Biosciences and Medicine
• Edition: 1
• Category: Library

✦ Synopsis

One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.

✦ Table of Contents

Front Matter....Pages I-X
Affinity-Based Separation: An Overview....Pages 1-15
Affinity Chromatography....Pages 16-28
Expanded Bed Affinity Chromatography....Pages 29-64
Perfusion Affinity Chromatography for Rapid Antibody Separations....Pages 65-81
Affinity-Based Interactions on Disks for Fast Analysis, Isolation and Conversion of Biomolecules....Pages 82-114
Sugars as Affinity Ligands....Pages 115-129
Macroaffinity Ligands in Bioseparation....Pages 130-147
Cellulose-Binding Fusion Proteins....Pages 148-162
Two-Phase Affinity Partitioning of Animal Cells: Implications of Multipoint Interactions....Pages 163-180
Affinity-Based Reverse Micellar Separations....Pages 181-194
Biosensors: An Emerging Tool in Bioanalytical Research — A Kinetic Analysis of the Analyte-Receptor Interactions in Biosensors....Pages 195-216
Back Matter....Pages 217-225

✦ Subjects

Analytical Chemistry; Biotechnology; Organic Chemistry; Biochemistry, general; Cell Biology