✦ LIBER ✦

Methods of Enzymatic Analysis || Glucose-6-phosphatase

✍ Scribed by Harper, Alfred E.

Book ID: 120444269
Publisher: Elsevier
Year: 1965
Weight: 307 KB
Category: Article
ISBN: 0123956307
DOI: 10.1016/B978-0-12-395630-9.50143-2

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A continuous spectrophotometric assay for glucose 6-phosphatase is described. The method uses glucose dehydrogenase and mutarotase as ancillary enzymes. Glucose 6-phosphatase activity is measured by following NADH formation at 340 nm. The method is linear, at least up to 38 mU in the test which corr