[Methods in Molecular Biology] In Vitro Mutagenesis Protocols Volume 634 || Random Mutagenesis Using a Mutator Strain

✍ Scribed by Braman, Jeff

• Book ID: 120179471
• Publisher: Humana Press
• Year: 2010
• Weight: 752 KB
• Category: Article
• ISBN: 1607616521
• DOI: 10.1007/978-1-60761-652-8_29

✦ Synopsis

Random mutagenesis is a useful technique to study the functions of different gene products. Propagation of the genes cloned in plasmids through a mutator strain, like Escherichia coli XL1-red, produces randomly mutagenized plasmid libraries. This method offers a very simple and economic way of introducing random point mutations throughout the gene with a fairly high mutation rate. The whole process involves transformation and propagation of a plasmid containing the desired gene into the XL-1 red strain, isolating the mutagenized plasmid library and transforming this library into a desired strain for screening the mutant phenotype.