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[Methods in Molecular Biology] In Vitro Mutagenesis Protocols Volume 634 || Insertion and Deletion Mutagenesis by Overlap Extension PCR

โœ Scribed by Braman, Jeff


Book ID
121398148
Publisher
Humana Press
Year
2010
Weight
170 KB
Category
Article
ISBN
1607616521

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Random mutagenesis is a useful technique to study the functions of different gene products. Propagation of the genes cloned in plasmids through a mutator strain, like Escherichia coli XL1-red, produces randomly mutagenized plasmid libraries. This method offers a very simple and economic way of intro