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[Methods in Enzymology] Synthetic Biology, Part B - Computer Aided Design and DNA Assembly Volume 498 || Multiplexed Genome Engineering and Genotyping Methods

โœ Scribed by Wang, Harris H.

Book ID
120339920
Publisher
Elsevier
Year
2011
Tongue
English
Weight
437 KB
Category
Article
ISBN
0123851203
ISSN
0076-6879

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[Methods in Enzymology] Synthetic Biolog
[Methods in Enzymology] Synthetic Biology, Part B - Computer Aided Design and DNA Assembly Volume 498 || Enzymatic Assembly of Overlapping DNA Fragments
โœ Gibson, Daniel G. ๐Ÿ“‚ Article ๐Ÿ“… 2011 ๐Ÿ› Elsevier ๐ŸŒ English โš– 306 KB

Three methods for assembling multiple, overlapping DNA molecules are described. Each method shares the same basic approach: (i) an exonuclease removes nucleotides from the ends of double-stranded (ds) DNA molecules, exposing complementary single-stranded (ss) DNA overhangs that are specifically anne