Methods in Cell Biology

✍ Scribed by Stephen M. King and Gregory J. Pazour (Eds.)

• Publisher: Academic Press
• Year: 2009
• Tongue: English
• Leaves: 333
• Series: Methods in Cell Biology 93
• Edition: 1
• Category: Library

✦ Synopsis

Cilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport (IFT) that is essential for the assembly and maintenance of ciliary structures. * Includes both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Covers forward and reverse genetic analysis of IFT and bochemical methods to define the role of IFT components * Methods presented cover molecular, genetic, and biochemical approaches to ciliary function in model organisms

✦ Table of Contents

Content:
Series Editors
Page ii

Volume Editors
Page iii

Copyright
Page iv

Contributors
Pages xi-xiii

Preface
Pages xv-xvi

Chapter 1 - Manipulating Ciliary Protein-Encoding Genes in Tetrahymena thermophila
Pages 1-20
Drashti Dave, Dorota Wloga, Jacek Gaertig

Chapter 2 - Approaches for Functional Analysis of Flagellar Proteins in African Trypanosomes
Pages 21-57
Michael Oberholzer, Miguel A. Lopez, Katherine S. Ralston, Kent L. Hill

Chapter 3 - Tools for Analyzing Intraflagellar Transport in Trypanosomes
Pages 59-80
Daria Julkowska, Philippe Bastin

Chapter 4 - Schmidtea mediterranea: A Model System for Analysis of Motile Cilia
Pages 81-98
Panteleimon Rompolas, Ramila S. Patel-King, Stephen M. King

Chapter 5 - Targeted Gene Silencing by RNA Interference in Chlamydomonas
Pages 99-110
Eun-Jeong Kim, Heriberto Cerutti

Chapter 6 - Analysis of Cargo Transport by IFT and GFP Imaging of IFT in Chlamydomonas
Pages 111-119
Dennis Diener

Chapter 7 - Genetic and Phenotypic Analysis of Flagellar Assembly Mutants in Chlamydomonas reinhardtii
Pages 121-143
Carlo Iomini, Jacob E. Till, Susan K. Dutcher

Chapter 8 - Recording and Analyzing IFT in Chlamydomonas Flagella
Pages 145-155
William Dentler, Kristyn VanderWaal, Mary E Porter

Chapter 9 - Total Internal Reflection Fluorescence (TIRF) Microscopy of Chlamydomonas Flagella
Pages 157-177
Benjamin D. Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B. Witman, Wallace F. Marshall

Chapter 10 - Purification of IFT Particle Proteins and Preparation of Recombinant Proteins for Structural and Functional Analysis
Pages 179-196
Robert H. Behal, Ewelina Betleja, Douglas G. Cole

Chapter 11 - Studying Cilia in Zebrafish
Pages 197-217
Iain Drummond

Chapter 12 - Analysis of IFT Kinesins in Developing Zebrafish Cone Photoreceptor Sensory Cilia
Pages 219-234
Christine Insinna, Katherine Luby-Phelps, Brian A. Link, Joseph C. Besharse

Chapter 13 - Analysis of Intraflagellar Transport in C. elegans Sensory Cilia
Pages 235-266
Limin Hao, Seyda Acar, James Evans, Guangshuo Ou, Jonathan M. Scholey

Chapter 14 - Functional Genomics of Intraflagellar Transport-Associated Proteins in C. elegans
Pages 267-304
Peter N. Inglis, Oliver E. Blacque, Michel R. Leroux

Chapter 15 - Generating Conditional Mutants to Analyze Ciliary Functions: The Use of Cre–Lox Technology to Disrupt Cilia in Specific Organs
Pages 305-330
Amber K. O’Connor, Robert A. Kesterson, Bradley K. Yoder

Chapter 16 - Imaging Intraflagellar Transport in Mammalian Primary Cilia
Pages 331-346
Tatiana Y. Besschetnova, Barnali Roy, Jagesh V. Shah

Chapter 17 - Analysis of Hedgehog Signaling in Mouse Intraflagellar Transport Mutants
Pages 347-369
Hyuk W. Ko, Aimin Liu, Jonathan T. Eggenschwiler

Index
Pages 371-381

Volume in Series
Pages 383-390

Color Plates
Pages C1-C16

✦ Subjects

Биологические дисциплины;Цитология;