Methods in Cell Biology

✍ Scribed by Stephen M. King and Gregory J. Pazour (Eds.)

• Publisher: Elsevier, Academic Press
• Year: 2009
• Tongue: English
• Leaves: 245
• Series: Methods in Cell Biology 92
• Edition: 1
• Category: Library

✦ Synopsis

Along with its companion volume on axonemal dynein-mediated motility, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella,  focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. * Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

✦ Table of Contents

Content:
Series Editors
Page ii

Volume Editors
Page iii

Copyright
Page iv

Contributors
Pages ix-xi

Preface
Pages xiii-xiv

Chapter 1 - Bioinformatic Approaches to Dynein Heavy Chain Classification
Pages 1-9
Toshiki Yagi

Chapter 2 - Identification and Characterization of Dynein Genes in Tetrahymena
Pages 11-30
David E. Wilkes, Nicole Bennardo, Clarence W.C. Chan, Yu-Loung Chang, Elizabeth O. Corpuz, Jennifer DuMond, Jordan A. Eboreime, Julianna Erickson, Jonathan Hetzel, Erin E. Heyer, Mark J. Hubenschmidt, Ekaterina Kniazeva, Hallie Kuhn, Michelle Lum, Andrea Sand, Alicia Schep, Oksana Sergeeva, Natt Supab, Caroline R. Townsend, Liesl Van Ryswyk, et al.

Chapter 3 - Purification of Axonemal Dyneins and Dynein-Associated Components from Chlamydomonas
Pages 31-48
Stephen M. King

Chapter 4 - Purification of Dyneins from Sperm Flagella
Pages 49-63
Kazuo Inaba, Katsutoshi Mizuno

Chapter 5 - Protein Engineering Approaches to Study the Dynein Mechanism using a Dictyostelium Expression System
Pages 65-82
Takahide Kon, Tomohiro Shima, Kazuo Sutoh

Chapter 6 - Biophysical Measurements on Axonemal Dyneins
Pages 83-105
Hiroaki Kojima, Shiori Toba, Hitoshi Sakakibara, Kazuhiro Oiwa

Chapter 7 - Protein Electroporation into Chlamydomonas for Mutant Rescue
Pages 107-111
Masahito Hayashi, Ritsu Kamiya

Chapter 8 - Analysis of the Role of Nucleotides in Axonemal Dynein Function
Pages 113-131
Chikako Shingyoji

Chapter 9 - The Regulation of Dynein-Driven Microtubule Sliding in Chlamydomonas Flagella by Axonemal Kinases and Phosphatases
Pages 133-151
Candice A. Elam, Winfield S. Sale, Maureen Wirschell

Chapter 10 - Analysis of Redox-Sensitive Dynein Components
Pages 153-161
Ken-ichi Wakabayashi

Chapter 11 - Calcium Regulation of Ciliary Motility: Analysis of Axonemal Calcium-Binding Proteins
Pages 163-180
Christen DiPetrillo, Elizabeth Smith

Chapter 12 - Isolation and Analysis of Radial Spoke Proteins
Pages 181-196
Pooja Kelekar, Mei Wei, Pinfen Yang

Chapter 13 - Analysis of the Central Pair Microtubule Complex in Chlamydomonas reinhardtii
Pages 197-213
David R. Mitchell, Brandon Smith

Index
Pages 215-221

Volume in Series
Pages 223-230

Color Plates
Pages C1-C8