<p><p>Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response. In <i>Protein Microarray
Methods for Protein Analysis: A Practical Guide for Laboratory Protocols
โ Scribed by Robert A. Copeland PhD (auth.)
- Publisher
- Springer US
- Year
- 1994
- Tongue
- English
- Leaves
- 238
- Edition
- 1
- Category
- Library
No coin nor oath required. For personal study only.
โฆ Synopsis
As protein science continues to become an increasingly important aspect of academic and commercial sciences and technology, the need has arisen for a ready source of laboratory protocols for the analysis and evaluation of these biological polymers. Methods for Protein Analysis presents the methods most relevant to the generalist bench scientist working with proteins. A concise yet thorough summary, it covers laboratory methods that can be reasonably performed in a standard protein laboratory, without specialized equipment or expertise. Taking a how to approach, this book examines the techniques used to answer common protein analytical questions and describes methods useful in daily laboratory work. Methods for Protein Analysis is the ideal reference for protein laboratories in academic, government and industrial settings. It is an essential benchtop manual for first-year graduate students beginning their laboratory experience as well as for chemists, biochemists, and molecular biologists in the pharmaceutical, biotechnological, food and specialty chemical industries, and for analysts concerned with the purity and structural integrity of protein. Featuring illustrations and a convenient spiral binding, this guide offers a glossary of common abbreviations and a list of suppliers for protein science.
โฆ Table of Contents
Front Matter....Pages i-xi
Introduction to Protein Structure....Pages 1-16
General Methods for Handling Proteins....Pages 17-38
Methods for Protein Quantitation....Pages 39-58
Electrophoretic and Chromatographic Methods for Assessing Protein Purity....Pages 59-98
Immunological Methods....Pages 99-112
Detection of Non-Protein Components....Pages 113-125
Peptide Mapping and Amino Acid Analysis....Pages 126-150
Residue-Specific Chemical Modification of Proteins....Pages 151-160
Spectroscopic Probes of Protein Structure....Pages 161-198
Protein Folding and Stability....Pages 199-216
Back Matter....Pages 217-228
โฆ Subjects
Biochemistry, general; Analytical Chemistry; Food Science; Pharmacology/Toxicology
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