Rapid Method for Determining the Rate of
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Itzhak Nissim; Stuart E. Starr; Kathleen E. Sullivan; Donald E. Campbell; Steven
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Article
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2000
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Elsevier Science
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English
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A new method has been developed for determination of DNA synthesis during cell proliferation. The method is based on the metabolism of [U-13 C 6 ]glucose to deoxyribose (DR) and then incorporation of [U-13 C 5 ]DR into newly synthesized DNA. Extracted cellular DNA is subjected to HCl hydrolysis (2 h