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Methods for Determining the Rates of Catalase Synthesis and Destruction in vivo

โœ Scribed by PRICE, V. E.; RECHCIGL, M.; HARTLEY, R. W.


Book ID
109613476
Publisher
Nature Publishing Group
Year
1961
Tongue
English
Weight
207 KB
Volume
189
Category
Article
ISSN
0028-0836

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A new method has been developed for determination of DNA synthesis during cell proliferation. The method is based on the metabolism of [U-13 C 6 ]glucose to deoxyribose (DR) and then incorporation of [U-13 C 5 ]DR into newly synthesized DNA. Extracted cellular DNA is subjected to HCl hydrolysis (2 h