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Methods and reagents: Reamplification of PCR fragments

✍ Scribed by Paul N. Hengen


Book ID
114326899
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
474 KB
Volume
20
Category
Article
ISSN
0968-0004

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In an effort to find a simple and inexpensive purification method of polymerase chain reaction (PCR) reaction before cycle sequencing reaction, we compared a commercial system with a precipitation protocol performed in our laboratory. We found that, particularly with small PCR products, our method w