✍ Scribed by Dr. Brigitte Glück; Axel Stelzner
No coin nor oath required. For personal study only.
The procedures generally used for the in vitro induction of interferon exploit fibroblast or leucocyte culture suspensions. Several factors and conditions influencing the capacity for IFN induction wefe studied, with the following results.
In contrast to previous findings, optimum IFN induction was obtained at 37 "C, rather than 30 "C, when human fibroblasts were induced with 100 pg/ml of Poly (IC). There was a direct correlation between the dose of the inducing agent applied and the amount of IFN produced. Also, the cells were excellently superinducible with metabolic inhibitors like actinomycin D and cycloheximine. In addition, priming procedures enhanced IFN induction in human fibroblasts.
Comparing different cell types with respect to their ability to be induced for IFN production, we found that different cell types show great differences in their inducer sensitivity. Finally, the response of different cell culture systems was highly variable when induced with different compounds.
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