Method for the quantitative evaluation of data from flow microfluorometry

✍ Scribed by Jerrold Fried

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 803 KB
• Volume: 9
• Category: Article
• ISSN: 0010-4809
• DOI: 10.1016/0010-4809(76)90006-9

✦ Synopsis

A new method is proposed for estimating the distribution of cells among the phases of the mitotic cycle using data from a flow microfluorometer. The method can be applied to asynchronous or synchronous cell populations, and is not limited to systems with very low coefficients of variation of fluorescence intensity. It is based on a mathematical model of the cell population having the following properties: (i) the population is separated into compartments, one consisting of G, cells, another of G, and M cells, and several of S phase cells which have synthesized different specified fractions of their DNA; (ii) the fluorescence intensity of cells in each compartment is normally distributed with the mean of the Gz + M compartment having a channel location twice that of G, ; the S phase compartments have means at intermediate values. Coefficients of variation are the same for all compartments. The sizes of the compartments are determined by a least squares fitting procedure utilizing the observed data. The method and some of its limitations are illustrated by selected examples of simulated data from asynchronous and partially synchronized cell populations.