Method for detection of 2-azahypoxanthine in human and rat urine

✍ Scribed by Diane D. Beal; George T. Bryan

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 379 KB
• Volume: 88
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90463-3

✦ Synopsis

A new, simple, reproducible, and quantitative method for the detection of 2azahypoxanthine (2-AH) in urine is described. The method is based on removal of interfering substances from urine by cation-and anion-exchange chromatography. Quantitative determination of 2-AH eluted from the anion-exchange resin involves its conversion to 5-diazoimidazole-4-carboxylic acid and subsequent coupling of this compound withN-(I-naphthyl)ethylenediamine.

The resultant dye product has an absorption maximum at 505 nm in 2.4 N HCI, which is linearly related to the original 2-AH concentration in the range of O-8 pg/ml. The only substance found to interfere and not be removed or destroyed is p-acetamidophenyl glucuronide, an excretion product of phenacetin-and acetaminophen-containing drugs.