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Methionine and iron as growth factors for rat embryos cultured in canine serum

✍ Scribed by Flynn, Thomas J. ;Friedman, Leonard ;Black, Thomas N. ;Klein, Norman W.

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 697 KB
Volume: 244
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402440216

No coin nor oath required. For personal study only.

✦ Synopsis

Development of headfold-staged rat embryos cultured in canine serum containing various supplements was compared with development in rat serum to seek suitable alternatives to rat serum in rodent embryo culture and to identify nutritional factors for cultured rodent embryos that may have relevance for normal mammalian embryonic growth and development. Supplementation of canine serum with glucose, methionine, and a lipophilic iron chelate allowed growth and development of cultured rat embryos, approximating those obtained with rat serum. These findings suggest that properly supplemented canine serum can serve as a suitable rodent embryo culture medium and that glucose, iron, and methionine may be important nutrients in mammalian embryonic development.

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