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Rapid induction of mRNAs for liver regeneration factor and insulin-like growth factor binding protein-1 in primary cultures of rat hepatocytes by hepatocyte growth factor and epidermal growth factor

✍ Scribed by Edward Weir; Qiuyan Chen; Marie C. Defrances; Aaron Bell; Rebecca Taub; Reza Zarnegar

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 689 KB
Volume: 20
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840200426

No coin nor oath required. For personal study only.

✦ Synopsis

Liver regeneration factor belongs to the leucinezipper family of transcription factors. It was originally cloned and characterized through differential screening of a regenerating rat liver cDNA library. The mRNA for liver regeneration factor-1 is barely detectable in normal rat liver but is dramatically induced after two-thirds hepatectomy, with a peak 1 to 3 hr after surgery. The nature of the signaling molecule(s) for this rapid induction is not known. It has been suggested that the liver regeneration factor-1 protein product, through complex interactions with other transcription factors such as c-Jun and Jun-B, controls expression of genes that are required during the G , phase of hepatic growth. Hepatocyte growth factor has been shown to be the most potent mitogen for hepatocytes in uitro and in uiuo. Plasma levels of hepatocyte growth factor rapidly (within 30 min) increase after loss of hepatic parenchyma induced by partial hepatectomy or carbon tetrachloride treatment. It has been postulated that hepatocyte growth factor plays a crucial role in stimulating the hepatocyte to enter the cell cycle. In this communication, we report that addition of pure hepatocyte growth factor to primary cultures of rat hepatocytes in the absence of serum and insulin results in rapid and transient induction of liver regeneration factor-1 mRNA (more than 20-fold) with a peak of expression 1 hr after treatment. The levels of jun-B and c-fos mRNAs, which are also known to be induced during the early hours of liver regeneration, were also increased after treatment of isolated hepatocytes with hepatocyte growth factor. Epidermal

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