A streptavidin-biotin-based three-step immunolabeling protocol for quantitative staining of intracellular antigens for flow cytometric analysis was evaluated using simian virus 40 (SV40) large T antigen. The concentration as well as the quantity of antibody used required optimization. The optimum la
Methanol fixation permits flow cytometric analysis of immunofluorescent stained intracellular antigens
β Scribed by Daniel Levitt; Marina King
- Book ID
- 118900794
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 630 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0022-1759
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