𝔖 Bobbio Scriptorium
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Flow cytometric analysis of antibody producing cells using double immunofluorescent staining

✍ Scribed by Gyun Min Lee; Jae Hoon Kim; Hee Gu Lee

Book ID
104647667
Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
414 KB
Volume
10
Category
Article
ISSN
0951-208X

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✦ Synopsis

Double fluorescent labeling, with fluorescein isothiocyanate (FIT0labeled F(ab')z specific lor the heavy chain and R-phycoerythrin (R-PE)-labeled F(ab')2 specific for the light chain, wiis demoilstrated :IS ;I convenient means for the accurate evaluation of a heterogeneous non-aIitibody-producing population. Furthermore, it could be used for monitoring the changes in each immunoglobulin (Ig) chain content of the cells during the batch culture, which will facilitate the study on antibody synthesis, xsemhly and secrerion.

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The expression of different proliferation associated nuclear antigens was analyzed using a washless double-staining method and flow cytometry. It is a simple and rapid two-step procedure which can be performed on low cell numbers. A series of hematopoietic cell lines and fresh lymphoma cells were te