𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Metallization of a Genetically Engineered Polypeptide

✍ Scribed by Autumn Carlsen; Seiichiro Higashiya; Natasha I. Topilina; Kathleen A. Dunn; Robert E. Geer; Eric T. Eisenbraun; Alain E. Kaloyeros; John T. Welch

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
450 KB
Volume
12
Category
Article
ISSN
1616-5187

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Recently, well‐ordered biological materials have been exploited to pattern inorganic nanoparticles into linear arrays that are of particular interest for nanoelectronic applications. In this work, a de novo designed E. coli‐expressed polypeptide (previously shown to form highly rectilinear, β‐sheet‐containing structures) operates as a template for divalent metal cations. EDX and TEM analysis verify the attachment of platinum ions to the histidine‐rich fibril surface, which was designed specifically to facilitate attachment of chemical moieties. Following chemical reduction, TEM further confirms the formation of localized zero‐valent metal aggregates with sub‐nanometer interparticle spacing.magnified image

📜 SIMILAR VOLUMES

Genetically engineered normal flora for
Genetically engineered normal flora for oral polypeptide delivery: Dose–absorption response
✍ Gagan Kaushal; Jun Shao 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 220 KB

Genetically modified Lactococcus lactis (L. lactis), a probiotic bacterium, able to secrete b-lactamase (29 kDa), was used as a vector for the oral delivery of b-lactamase to the rats. Three different doses of L. lactis were administered to the rats, and the resulted b-lactamase oral bioavailability

Optical Determination of Glutamine Using
Optical Determination of Glutamine Using a Genetically Engineered Protein
✍ Jonathan D. Dattelbaum; Joseph R. Lakowicz 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 201 KB

We have developed a reagentless optical biosensor for glutamine based on the Escherichia coli glutamine binding protein (GlnBP). Site-directed mutagenesis was performed to engineer single cysteine mutants which were covalently modified with environmentally sensitive sulfhydryl-reactive probes. The f

Genetically engineered HSV in the treatm
Genetically engineered HSV in the treatment of glioma: a review
✍ James M. Markert; G. Yancey Gillespie; Ralph R. Weichselbaum; Bernard Roizman; R 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 311 KB 👁 1 views

Central nervous system malignancies, particularly glioblastoma multiforme, pose signi®cant problems for the development of novel therapeutics. In the absence of advances with standard surgical and chemotherapeutic approaches, the utilisation of genetically engineered viruses, both as direct oncolyti