Metallic Zinc Reduction of Disulfide Bonds between Cysteine Residues in Peptides and Proteins
✍ Scribed by Mikael Erlandsson; Mattias Hällbrink
- Publisher
- Springer Netherlands
- Year
- 2005
- Tongue
- English
- Weight
- 178 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1573-3149
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A simple, rapid, and quantitative procedure for the protection of reduced S-S bonds in proteins by selective methylation with dimethylsuifate is described. The formed S-methylcysteine is a stable derivative well suited for ion-exchange chromatographic (iec) or gas-liquid chromatographic (glc) analys
Tandem mass spectrometry employing high-energy, collisionally activated dissociation (CAD) is shown to be a useful method for sequencing through the cystine bridge of intermolecularly disulfide-bonded peptides. A characteristic triplet of intense fragment ions is observed corresponding to cleavage t