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Metal Phosphonates Applied to Biotechnologies: A Novel Approach to Oligonucleotide Microarrays

✍ Scribed by Bruno Bujoli; Sarah M. Lane; Guillaume Nonglaton; Muriel Pipelier; Jean Léger; Daniel R. Talham; Charles Tellier

Book ID
102793034
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
666 KB
Volume
11
Category
Article
ISSN
0947-6539

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✦ Synopsis

Abstract

A new process for preparing oligonucleotide arrays is described that uses surface grafting chemistry which is fundamentally different from the electrostatic adsorption and organic covalent binding methods normally employed. Solid supports are modified with a mixed organic/inorganic zirconium phosphonate monolayer film providing a stable, well‐defined interface. Oligonucleotide probes terminated with phosphate are spotted directly on to the zirconated surface forming a covalent linkage. Specific binding of terminal phosphate groups with minimal binding of the internal phosphate diesters has been demonstrated. The mixed organic/inorganic thin films have also been extended for use arraying DNA duplex probes, and therefore represent a viable general approach to DNA‐based bioarrays. Ideas for interfacing mixed organic/inorganic interfaces to other bioapplications are also discussed.

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## Abstract Five new lead(II) sulfonate‐phosphonates, namely [Pb~3~(L)~2~(H~2~O)~2~]·4H~2~O (1), [Pb(HL)(phen)]·H~2~O (2), [Pb~6~(L)~4~(phen)~8~]·3H~2~O (3), [Pb~6~(L)~4~(phen)~10~]·2H~2~O (4), and [Pb~6~(L)~4~(4,4′‐bipy)(H~2~O)~2~]·2H~2~O (5; H~3~L = __m__‐HO~3~S‐C~6~H~4~‐PO~3~H~2~, phen = 1,10‐ph