Metal-Induced sequential transitions among DNA conformations

✍ Scribed by Yong A. Shin; James J. Butzow; Lisa D. Sinsel; Patricia Clark; Rajasekharan P. Pillai; W. Curtis Johnson; Gunther L. Eichhorn

• Book ID: 102762388
• Publisher: Wiley (John Wiley & Sons)
• Year: 1988
• Tongue: English
• Weight: 943 KB
• Volume: 27
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360270908

✦ Synopsis

The action of [Co(NH,),]Cl, on poly (dGdC) . poly(dGdC) can lead to a Series of consecutive reactions, in which B-DNA is first converted to Z-DNA [M. Behe and G. Felsenfeld (1981) Proc.

Natl. A d . Sci USA 78,1619-16231, which in turn is transformed into an unidentified conformer that we tentatively call " U , and finally the highly associated anisotropic +-DNA is produced.

Conditions are given under which the sequence B * Z * "U" * +( +) can be stopped at any point in the direction from left to right. The reverse processes, from right to left, occur when $(+) or "U"-DNA are treated with various amounts of salt concentrations and lowering temperature. Thus it is demonstrated that four conformers of poly(dGdC) . poly(dGdC) are readily interconvertible, and that Z-DNA and " U conformers are intermediates in the reversible transformations of B-and $-DNA.