Metabolism and chemical activation ofPhycomyces blakesleeanus spores
✍ Scribed by Andre J. Van Laere; J´ozef A. Van Assche; Albert R. Carlier
- Book ID
- 119078309
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 886 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0147-5975
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Heat treatment of Phycomyces sporangiospores, which breaks dormancy, causes a very rapid 10- to 15fold increase in trehalase activity; soon after the heat shock the enzyme activity decays. This phenomenon can be repeated several times by repeating the heat shocks. Prolonging the heat treatment over
Evidence is obtained for the existence of two different localizations of trehalase (α,α-trehalose glucohydrolase, EC 3.2.1.28) in Phycomyces spores: one inside the cell, and one in the periplasmic region. The latter enzyme is sensitive to 0.1 mol l(-1) HCl treatment and its activity can be regulated
The metabolism of [(14)C]glucose has been studied in Phycomyces spores during dormancy, activation, and the initial stages of germination. Dormant spores are able to take up and metabolize exogenous glucose into different products; the major part of it goes to trehalose synthesis (up to 60% when the
A great deal of effort has gone into research on the mechanisms of action of chemical carcinogens. The action of the host on the carcinogen represents one approach--to determine what metabolic products may be involved. Study of the action of the carcinogen on the host requires a thorough comparison