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Trehalase activity in dormant and activated spores ofPhycomyces blakesleeanus

✍ Scribed by J. A. Assche; A. R. Carlier; Helena I. Dekeersmaeker

Publisher: Springer-Verlag
Year: 1972
Tongue: English
Weight: 360 KB
Volume: 103
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00386704

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✦ Synopsis

Heat treatment of Phycomyces sporangiospores, which breaks dormancy, causes a very rapid 10- to 15fold increase in trehalase activity; soon after the heat shock the enzyme activity decays. This phenomenon can be repeated several times by repeating the heat shocks. Prolonging the heat treatment over the minimum required time delays the decay of enzyme activity. Cycloheximide does not prevent the rise in enzyme activity. It is suggested that heat treatment converts temporarily an inactive form of trehalase into an active one. Optimal enzyme activity is obtained at pH 7.5 and the enzyme requires metal ions for maximal activity. The possible role of trehalase in the spore-activation process is discussed.

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