Metabolic flux analysis of CHO cell metabolism in the late non-growth phase
β Scribed by Neelanjan Sengupta; Steven T. Rose; John A. Morgan
- Book ID
- 102763194
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 833 KB
- Volume
- 108
- Category
- Article
- ISSN
- 0006-3592
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## Abstract Chinese hamster ovary (CHO) cells are the most widely used mammalian cell line for biopharmaceutical production, with a total global market approaching $100 billion per year. In the pharmaceutical industry CHO cells are grown in fedβbatch culture, where cellular metabolism is characteri
Metabolic flux analysis is a useful tool to analyze cell metabolism. In this study, we report the use of a metabolic model with 34 fluxes to study the 293 cell, in order to improve its growth capacity in a DMEMΓF12 medium. A batch, fed-batch with glutamine feeding, fed-batch with essential amino aci