## Abstract Chinese hamster ovary (CHO) cells are the most widely used mammalian cell line for biopharmaceutical production, with a total global market approaching $100 billion per year. In the pharmaceutical industry CHO cells are grown in fedβbatch culture, where cellular metabolism is characteri
β¦ LIBER β¦
Investigation of glutamine metabolism in CHO cells by dynamic metabolic flux analysis
β Scribed by Wahrheit, Judith; Nicolae, Averina; Heinzle, Elmar
- Book ID
- 121591498
- Publisher
- BioMed Central
- Year
- 2013
- Tongue
- English
- Weight
- 84 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1753-6561
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Metabolic flux analysis is a useful tool to analyze cell metabolism. In this study, we report the use of a metabolic model with 34 fluxes to study the 293 cell, in order to improve its growth capacity in a DMEMΓF12 medium. A batch, fed-batch with glutamine feeding, fed-batch with essential amino aci
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