Metabolic Fate of 14C-Labeled Glutamate in Astrocytes in Primary Cultures
β Scribed by Albert C. Yu; Arne Schousboec; Leif Hertz
- Book ID
- 118713955
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 753 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0022-3042
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π SIMILAR VOLUMES
The metabolic fate of L-[ ''C]-glutamine was followed in cerebral cortical neurons in primary cultures, a GABAergic preparation. Part of the glutamine was converted to GABA (0.3 nmolimin per mg protein), which is consistent with the presence of glutaminase and glutamate decarboxylase activity in the
## Abstract The effect of Ξ³βaminobutyric acid (GABA) on glutamate metabolism was studied by ^13^Cβnuclear magnetic resonance (NMR) spectroscopy. Cerebral cortical astrocytes were incubated with 0.5 mM [Uβ^13^C]glutamate and 5 mM glucose in the presence or absence of 0.2 mM GABA for 2 hr. ^13^CβLabe