✦ LIBER ✦

Metabolic fate of [14C]-glutamine in mouse cerebral neurons in primary cultures

✍ Scribed by A. C. H. Yu; T. E. Fisher; E. Hertz; J. T. Tildon; A. Schousboe; Dr. L. Hertz

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 458 KB
Volume: 11
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490110403

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✦ Synopsis

The metabolic fate of L-[ ''C]-glutamine was followed in cerebral cortical neurons in primary cultures, a GABAergic preparation. Part of the glutamine was converted to GABA (0.3 nmolimin per mg protein), which is consistent with the presence of glutaminase and glutamate decarboxylase activity in the cells and with findings by other authors in vivo or in brain slices. However, an even larger part (1.8 nmol/min per mg protein) was converted to COz and succinate via an oxidative deamination to a-ketoglutarate. This is not consistent with the conccpt that transfer of glutamine from astrocytes to neurons should replenish neuronal GABA stores quantitatively after release of GABA and its partial accumulation into astrocytes, but it is well compatible with the recent demonstration of a net glutaniine uptake by the brain.

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