Mercuric Ion Inhibits the Activity and Fidelity of the Human Cell DNA Synthesome
β Scribed by Jennifer W. Sekowski; Linda H. Malkas; Yuetong Wei; Robert J. Hickey
- Book ID
- 112266449
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 436 KB
- Volume
- 145
- Category
- Article
- ISSN
- 0041-008X
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## Abstract We previously reported on the purification and characterization of a functional multiβprotein DNA replication complex (the DNA synthesome) from human cells and tissues. The synthesome is fully competent to carryβout all phases of the DNA replication process in vitro. In this study, DNA
Heavy metals, including zinc (Zn) and cadmium (Cd), are potentially important genotoxic agents in our environment. Here we report that human DNA ligase I, the major form of the enzyme in replicative cells, is a target for Zn and Cd ions. ZnCl2 at 0.8 mM caused complete inhibition of DNA ligase I act