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Mendelian inheritance of streptomycin resistance inNicotiana plumbaginifolia

✍ Scribed by Yen-Yu Kao; Chiu-Yueh Hung; Kin-Ying To; Chi-Chang Chen

Publisher: Springer
Year: 1991
Tongue: English
Weight: 536 KB
Volume: 10
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00232607

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✦ Synopsis

In a previous study two haploid streptomycin-resistant clones of Nicotiana plumbaginifolia were isolated. The chromosome number of one of these clones has now been doubled through leaf-midvein culture and the resultant diploids were characterized genetically. Our results show that streptomycin resistance in this clone is conditioned by a recessive nuclear gene. Haploid protoplasts of this streptomycin-resistant mutant were selected for chlorate resistance. All clones obtained from the selection were deficient in nitrate reductase activity in addition to resistance to streptomycin. Genetic analysis of progeny of one of these clones revealed that the genes for streptomycin resistance and for the apoenzyme of nitrate reductase are unlinked.

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