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Streptomycin resistance is inherited as a recessive Mendelian trait in aNicotiana sylvestrisline

✍ Scribed by P. Maliga


Publisher
Springer
Year
1981
Tongue
English
Weight
700 KB
Volume
60
Category
Article
ISSN
0040-5752

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✦ Synopsis


The SR180 cell line has been isolated in a callus culture derived from a haploid Nicotiana sylvestris (n = X = 12) plant by its ability to proliferate on a selective medium containing 2,000 μg/ml streptomycin sulphate. From the cell line diploid plants have been regenerated. The SR180 selfs are resistant to streptomycin. Streptomycin sensitivity in F1, and a 3∶1 (sensitive to resistant) segregation in F2 indicate that resistance in the SR180 mutant is the result of a recessive Mendelian mutation.