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Membrane Potential Estimation by Flow Cytometry

✍ Scribed by Howard M. Shapiro

Book ID
115639179
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
86 KB
Volume
21
Category
Article
ISSN
1046-2023

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Mitochondrial membrane potential measure
Mitochondrial membrane potential measurement in rat cerebellar neurons by flow cytometry
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Mitochondrial membrane potential (MMP) in dissociated rat cerebellar neurons was measured using rhodamine 123 (Rh 123) as fluorescent dye, and flow cytometry. Dye distribution was studied by confocal scanning microscopy. Propidium iodide (PI)-marked cells (dead cells) were not stained by Rh 123, whi