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Membrane potential changes during IgE-mediated histamine release from rat basophilic leukemia cells

✍ Scribed by Ronit Sagi-Eisenberg; Israel Pecht

Publisher
Springer
Year
1983
Tongue
English
Weight
720 KB
Volume
75
Category
Article
ISSN
0022-2631

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✍ Shoko Iwaki; Masahito Ogasawara; Ryoji Kurita; Osamu Niwa; Katsuyuki Tanizawa; Y πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 103 KB

To detect low levels of histamine, we developed a histamine microsensor using recombinant histamine oxidase. Histamine oxidase with a histidine tag was readily purified using a histidine affinity column. The enzyme showed higher catalytic activity on histamine than diamines (e.g., putrescine and cad