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IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones

โœ Scribed by Edward L. Barsumian; Chaviva Isersky; Marianne G. Petrino; Reuben P. Siraganian

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
826 KB
Volume
11
Category
Article
ISSN
0014-2980

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Real-Time Monitoring of Histamine Releas
Real-Time Monitoring of Histamine Released from Rat Basophilic Leukemia (RBL-2H3) Cells with a Histamine Microsensor Using Recombinant Histamine Oxidase
โœ Shoko Iwaki; Masahito Ogasawara; Ryoji Kurita; Osamu Niwa; Katsuyuki Tanizawa; Y ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 103 KB

To detect low levels of histamine, we developed a histamine microsensor using recombinant histamine oxidase. Histamine oxidase with a histidine tag was readily purified using a histidine affinity column. The enzyme showed higher catalytic activity on histamine than diamines (e.g., putrescine and cad