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Membrane-mediated capillary electrophoresis: Interaction of cationic peptides with bicelles

✍ Scribed by John O. Mills; Lisa A. Holland

Publisher: John Wiley and Sons
Year: 2004
Tongue: English
Weight: 84 KB
Volume: 25
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.200405879

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✦ Synopsis

Electrokinetic capillary chromatography is applied to determine the membrane affinity of peptides using both 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) micelles and DHPC/1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bicelles under controlled conditions. The effect of temperature and the bicelle q value in surface association with cationic peptides is studied. The cationic peptides selected have a well-defined membrane structure (indolicidin), induced secondary structure (melittin, magainin 2), or do not possess classical secondary structure (atrial natriuretic peptide (ANP) 1-28, 4-28, 5-27). Electrokinetic capillary chromatography facilitated by DMPC and DHPC additives provides a rapid means of estimating lipophilicity and screening for peptides that have membrane affinity.

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