Membrane Features and Activity of GPI-Anchored Enzymes: Alkaline Phosphatase Reconstituted in Model Membranes †
✍ Scribed by Sesana, Silvia; Re, Francesca; Bulbarelli, Alessandra; Salerno, Domenico; Cazzaniga, Emanuela; Masserini, Massimo
- Book ID
- 126261417
- Publisher
- American Chemical Society
- Year
- 2008
- Tongue
- English
- Weight
- 736 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0006-2960
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## Abstract In plasma membranes, most of glycosylphosphatidylinositol (GPI)‐anchored proteins would be associated with rafts, a category of ordered microdomains enriched in sphingolipids and cholesterol (Ch). They would be also concentrated in the detergent resistant membranes (DRMs), a plasma memb
Treatment of liver plasma membranes with Triton X-100 allowed an endogenous alkaline phosphatase-converting activity to convert amphiphilic alkaline phosphatase (membrane anchor covalantly attached) to hydrophilic dimers that resemble the enzyme found in normal plasma. The Triton-solubilized activit