Membrane effects of the polyene antibiotic amphotericin B and of some of its derivatives on lymphocytes

✍ Scribed by N. Henry-Toulmé; P. Sarthou; M. Seman; J. Bolard

• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 444 KB
• Volume: 91
• Category: Article
• ISSN: 0300-8177
• DOI: 10.1007/bf00228077

✦ Synopsis

Amphotericin B (AmB) exhibits immunomodulating properties in mice. In vitro studies on lymphocytes, in relation with these properties, are reported here with AmB and two of its derivatives: the N-Fructosyl (N-Fru AmB) and the N-thiopropionyl (AmBSH) derivatives. Interactions of these molecules with thymocytes, a sensitive cell type, demonstrated that the extent of binding is not a toxicity parameter. In contrast, membrane fluidity changes have been observed and appeared to be related to toxicity. Experiments performed with normal B lymphocytes have shown that Amphotericin B derivatives were more potent polyclonal B cell activators than the parent compound. To go further in the understanding of these events, we have investigated in a B cell line WEHI 231, the changes in intracellular Ca2+ and membrane potential induced by AmB and AmBSH. The two polyenes were shown to induce membrane depolarization but no intracellular Ca2+ increase.