Membrane-Based Affinity Technology for Commercial Scale Purifications
β Scribed by Brandt, Steve ;Goffe, Randal A. ;Kessler, Stephen B. ;O'Connor, James L. ;Zale, Stephen E.
- Book ID
- 109905518
- Publisher
- Nature Publishing Group
- Year
- 1988
- Tongue
- English
- Weight
- 696 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1087-0156
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## Abstract Membrane systems are used throughout the downstream purification in the commercial production of high value therapeutic proteins. Over the last two decades, new membranes, modules, and systems have been developed specifically to meet the requirements of the biotechnology industry. These
N-Acetyl-D-(-)-penicillamine as a stable second-generation biospecific affinity ligand has previously been suggested for purification of Bacillus cereus 569/H beta-lactamase I. A complex spacer arm is coupled with the matrix by using epichlorohydrin and phloroglucinol doubly activated with divinyl s