Mediated amperometric biosensor for nicotinic acid based on whole cells of Pseudomonas fluorescens

✍ Scribed by Katsumi Takayama; Tokuji Ikeda; Toru Nagasawa

• Book ID: 102179979
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 425 KB
• Volume: 8
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.1140080811

✦ Synopsis

Whole cells of Psrudomonaspuorescrns TN5 were immobilized on the surface of carbon paste electrodes containing p-benzoquinone or its derivatives. The electrodes produced anodic currents for nicotinic acid due to the electrocatalytic action of the bacterial cells using the quinone compounds as an electron transfer mediator. The characteristics as a nicotinic acid sensor were studied of the electrode containing p-benzoquinone. It could measure nicotinic acid in the range 5pM to 6mM with a response time of 80s and with high selectivity in the solution of pH between 4 and 10. The electrode was stable for at least two weeks when used for the measurment of nicolinic acid at the concentration below I mM. An alternative method of measuring nicotinic acid. which is based on the respiration reaction of P. jlziorrscrns, is also described.