𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite

✍ Scribed by Arnhold, J. ;Mueller, S. ;Arnold, K. ;Sonntag, K.

Publisher
John Wiley and Sons
Year
1993
Weight
533 KB
Volume
8
Category
Article
ISSN
0884-3996

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Two different mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite were found. Most substances investigated in these experiments acted by scavenging NaOCI. This mechanism was independent of the concentration of hydrogen peroxide and the incubation time between luminol and inhibitors. The most potent inhibitors were substances containing SH groups. Compounds with amino groups as a target for HOCI/OCI^βˆ’^ to yield chloramines were much less effective inhibitors. Another mechanism of inhibition was found for catalase. It depended on the presence of hydrogen peroxide in the incubation medium and the incubation time between luminol and catalase. The enzyme inhibited the luminescence by removing H~2~O~2~ at molar concentrations much smaller than those found for all other inhibitors. Our results confirm the present models of the mechanism of generation of luminescence in luminol oxidation.

πŸ“œ SIMILAR VOLUMES

Chemiluminescence intensities and spectr
Chemiluminescence intensities and spectra of luminol oxidation by sodium hypochlorite in the presence of hydrogen peroxide
✍ Arnhold, J. ;Mueller, S. ;Arnold, K. ;Grimm, E. πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons βš– 321 KB πŸ‘ 2 views

Hydrogen peroxide amplifies the chemiluminescence in the odixation of luminol by sodium hypochlorite. A linear relationship between concentration of hydrogen peroxide and light intensity was found in the concentration range 5 x 10-\*-7.5 x mol/l. At 7.5 x l o -' mol/l H,Oz the chemiluminescence is a

Enhancement and inhibition of luminol ch
Enhancement and inhibition of luminol chemiluminescence by phenolic acids
✍ DΓ­az, A. Navas ;SΓ‘nchez, F. GarcΓ­a ;GarcΓ­a, J. A. GonzΓ‘Lez πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons βš– 603 KB

We explored the behaviour o f a series o f phenolic acids used as enhancers or inhibitors of luminol chemiluminescence by three different methods t o determine if behaviour was associated with phenolic acid structure and redox character. All the phenolic acids inhibited chemiluminescence when hexacy

Kinetics and mechanism of oxidation of s
Kinetics and mechanism of oxidation of starch with sodium hypochlorite
✍ K. F. Patel; H. U. Mehta; H. C. Srivastava πŸ“‚ Article πŸ“… 1974 πŸ› John Wiley and Sons 🌐 English βš– 542 KB

Kinetics of heterogeneous oxidation of starch with hypochlorite were studied under varying conditions of temperature, pH, buffer components, buffer concentration, and concentration of substrate and oxidant. The kinetic data show that the reaction is first order with respect to hypochlorite. A plot o

Inhibition of monocyte luminol-dependent
Inhibition of monocyte luminol-dependent chemiluminescence by tetrahydrobiopterin, and the free radical oxidation of tetrahydrobiopterin, dihydrobiopterin and dihydroneopterin
✍ Simon J. R. Heales; John A. Blair; Christian Meinschad; Irmgard Ziegler πŸ“‚ Article πŸ“… 1988 πŸ› John Wiley and Sons 🌐 English βš– 336 KB

Luminol-dependent chemiluminescence of normal human monocytes activated by zymosan is demonstrated to be inhibited by tetrahydrobiopterin in a concentration-dependent manner. The reduced pterins tetrahydrobiopterin, dihydrobiopterin, and dihydroneopterin are all shown to be readily oxidized by the h