Mechanism of integration and excision in conjugative transposons
β Scribed by P. Mullany; A. P. Roberts; H. Wang
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 436 KB
- Volume
- 59
- Category
- Article
- ISSN
- 1420-682X
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We have demonstrated that precise excision of bacterial transposon Tn5 can occur in the yeast, Saccharomyces cerevisiae. Tn5 insertions in the yeast gene LYS2 were generated by transposon mutagenesis made in Escherichia coli by means of a lambda::Tn5 vector. Nine insertions of Tn5 into the structura
## Abstract A major problem in gene therapy is the determination of the rates at which gene transfer has occurred. Our work has focused on applications of the __Sleeping Beauty__ (SB) transposon system as a nonβviral vector for gene therapy. Excision of a transposon from a donor molecule and its in