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Mechanism of high-copy-number integration of pMIRY-type vectors into the ribosomal DNA of Saccharomyces cerevisiae

✍ Scribed by Lopes, Teresa S.; Hakkaart, Gert-Jan A.J.; Koerts, Bert L.; Raué, Hendrik A.; Planta, Rudi J.


Book ID
122759086
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
917 KB
Volume
105
Category
Article
ISSN
0378-1119

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Yeast vectors suitable for high-level expression of heterologous proteins should combine a high copy number with high mitotic stability. The pMIRY integrative vector system. based upon targeted integration into the yeast rDNA locus, developed in our laboratory satisfies these criteria. However, inse