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High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression

✍ Scribed by Teresa S. Lopes; Jacobus Klootwijk; Annemarie E. Veenstra; Paul C. van der Aar; Harm van Heerikhuizen; Hendrik A. Raué; Rudi J. Planta


Book ID
118203886
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
702 KB
Volume
79
Category
Article
ISSN
0378-1119

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Yeast vectors suitable for high-level expression of heterologous proteins should combine a high copy number with high mitotic stability. The pMIRY integrative vector system. based upon targeted integration into the yeast rDNA locus, developed in our laboratory satisfies these criteria. However, inse