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Mechanism and stereochemistry of .alpha.,.beta.-dihydroxyacid dehydratase

โœ Scribed by Pirrung, Michael C.; Holmes, Christopher P.; Horowitz, Daniel M.; Nunn, David S.


Book ID
127044560
Publisher
American Chemical Society
Year
1991
Tongue
English
Weight
728 KB
Volume
113
Category
Article
ISSN
0002-7863

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The reaction catalyzed by bacterial GDP-mannose dehydratase (E.C. 4.2.1.47), the conversion of GDP-D-mannose to GDP-4-keto-6-deoxymannose (GDP-6-deoxy-D-lyxo-hexos-4-ulose), was studied with (6R)- and (6S)-GDP-D-[4-2H1,6-3H]mannose. Conversion of these stereospecifically labeled substrates in the pr