A technique for determining the amount of thermally denatured, insoluble protein is described. The assay has been validated using four globular proteins, bovine serum albumin, /Ilactoglobulin, lysozyme, and ovalbumin. It consists of a resolubilization protocol, using 8 M urea and 5% 2-mercaptoethano
Measuring plant protein with the Bradford assay
โ Scribed by Clive G. Jones; J. Daniel Hare; Steve J. Compton
- Book ID
- 105038833
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 787 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0098-0331
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A procedure was developed for the quantitation of solubilized proteins using the Bradford assay in the presence of glucopyranoside detergents. These detergents solubilized membranebound proteins with minimal background absorbance at 595 nm. Absorbance at 650 nm was also low, indicating that these de